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Article

A critical role for highly conserved Glu(610) residue of oligopeptidase B from Trypanosoma brucei in thermal stability.

Journal of biochemistry - 1 Feb 2010

Mohd Ismail Nor Ismaliza, Yuasa Tsuyoshi, Yuasa Keizo, Nambu Yuko, Nisimoto Makoto, Goto Masaki, Matsuki Hitoshi, Inoue Masahiro, Nagahama Masami, Tsuji Akihiko

Abstract excerpt

Oligopeptidase B from Trypanosoma brucei (Tb OPB) is a virulence factor and therapeutic target in African sleeping sickness. Three glutamic acid residues at positions 607, 609 and 610 of the catalytic domain are highly conserved in the OPB subfamily. In this study, the roles of Glu(607), Glu(609) and Glu(610) in Tb OPB were investigated by site-directed mutagenesis. A striking effect on k(cat)/K(m) was obtained...

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PMID: 19819899
DOI: 10.1093/jb/mvp156

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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