Article
The role of amino-acid residues Q39 and E451 in the determination of substrate specificity of the Spodoptera frugiperda beta-glycosidase.
European journal of biochemistry - 1 Aug 2002
Marana Sandro R, Terra Walter R, Ferreira Clélia
Abstract excerpt
The design of beta-glycosidases with planed substrate specificity for biotechnological application has received little attention. This is mostly a consequence of the lack of data on the molecular basis of the beta-glycosidase specificity, namely data on the energy of the noncovalent interactions in the enzyme-transition state complex. In an attempt to fill this gap, site-directed mutagenesis and enzyme...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Sequence Homology, Amino Acid
- Spodoptera
- Substrate Specificity
- beta-Glucosidase