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Article

The effect of specific proline residues on the kinetic stability of the triosephosphate isomerases of two trypanosomes.

Proteins - 1 Apr 2017

Guzmán-Luna Valeria, Quezada Andrea G, Díaz-Salazar A Jessica, Cabrera Nallely, Pérez-Montfort Ruy, Costas Miguel

Abstract excerpt

The effect of specific residues on the kinetic stability of two closely related triosephosphate isomerases (from Trypanosoma cruzi, TcTIM and Trypanosoma brucei, TbTIM) has been studied. Based on a comparison of their β-turn occurrence, we engineered two chimerical enzymes where their super secondary β-loop-α motifs 2 ((βα)2 ) were swapped. Differential scanning calorimetry (DSC) experiments showed that the (βα)2...

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Identifiers

PMID: 28002620
DOI: 10.1002/prot.25231

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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