Article
Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine.
Biochimie - 1 Aug 2017
Mikhailova Anna G, Rakitina Tatiana V, Timofeev Vladimir I, Karlinsky David M, Korzhenevskiy Dmitry A, Agapova Yulia К, Vlaskina Anna V, Ovchinnikova Marina V, Gorlenko Valentina A, Rumsh Lev D
Abstract excerpt
Oligopeptidase B (OpdB; EC 3.4.21.83) is a trypsin-like peptidase belonging to the family of serine prolyl oligopeptidases; two-domain structure of the enzyme includes C-terminal peptidase catalytic domain and N-terminal seven-bladed β-propeller domain. Importance of the interface between these domains and particularly of the 5 salt bridges for enzyme activity was established for protozoan OpdBs. However, these...
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