Article

Optimized Workflow for Self-Delivering siRNA-Mediated Gene Knockdown in Unstimulated Human CD4+ T Cells

2025-07-16

Abstract excerpt

T-cell-based therapies have transformed cancer treatment, yet dissecting the molecular networks that govern T-cell function remains technically challenging. Gene manipulation in primary human T cells typically relies on electroporation or viral vectors, approaches that often compromise viability, alter cell physiology, or introduce genotoxic risks. Accell™ small interfering RNAs (siRNAs) are chemically modified, s...

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Literature Corpus work: 5cc93670-b873-53f9-a83e-385a475c61da
DOI: 10.1101/2025.07.11.664322

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