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Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures

26 May 2021

Abstract excerpt

Differentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of...

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PMID: 34037474
DOI: 10.1152/ajplung.00122.2021

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