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Article

An optimized method for gene knockdown in differentiating human and mouse adipocyte cultures

2023-12-15

Abstract excerpt

Adipocyte cultures are a mainstay of metabolic disease research, yet loss-of-function studies in differentiating adipocytes is complicated by the refractoriness of lipid-containing adipocytes to standard siRNA transfections. Alternative methods, such as electroporation or adenovirus/lentivirus-based delivery systems are complex, expensive and often accompanied with unacceptable levels of cell death. To address thi...

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Identifiers and source

Literature Corpus work
83edd883-594d-5cbd-aedb-f73a21787aa3
DOI
10.1101/2023.12.14.571780
Open publication

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An optimized method for gene knockdown in differentiating human and mouse adipocyte culturesDOI 10.1101/2023.12.14.571780
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