Article
Development of a new Geobacillus lipase variant GDlip43 via directed evolution leading to identification of new activity-regulating amino acids.
International journal of biological macromolecules - 15 May 2020
Druteika Gytis, Sadauskas Mikas, Malunavicius Vilius, Lastauskiene Egle, Taujenis Lukas, Gegeckas Audrius, Gudiukaite Renata
Abstract excerpt
In this study three lipases GD-28, GD-95 and GD-66 (all 43 kDa in size), isolated from Geobacillus spp. were subjected to directed evolution experiments to yield a new synthetic lipolytic enzyme. This new lipase, obtained by DNA shuffling and epPCR, was named GDlip43 (also 43 kDa in size). It demonstrated increased thermoactivity, thermostability, an ability to hydrolyze short and long acyl chain p-NP esters and...
Topics
- Amino Acids
- Chemical Phenomena
- Cloning, Molecular
- Computational Biology
- Enzyme Stability
- Evolution, Molecular
- Gene Expression
- Genetic Variation
- Geobacillus
- Hydrogen-Ion Concentration
- Lipase
- Models, Molecular