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Article

Genetic analysis of the interaction between bacteriophage T7 DNA polymerase and Escherichia coli thioredoxin.

Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 1992

Himawan J S, Richardson C C

Abstract excerpt

Gene 5 protein of bacteriophage T7 is a nonprocessive DNA polymerase. During infection of Escherichia coli, T7 annexes the host protein thioredoxin for use as a processivity factor for T7 DNA polymerase. We describe here a genetic method to investigate the interaction between T7 gene 5 protein and E. coli thioredoxin. The strategy is to use thioredoxin mutants that are unable to support the growth of wild-type T7...

Topics

DNA Replication
DNA-Directed DNA Polymerase
Escherichia coli
Genes, Suppressor
Genetic Complementation Test
Macromolecular Substances
Mutagenesis, Site-Directed
Mutation
Protein Structure, Tertiary
Structure-Activity Relationship

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

T-Phages

Thioredoxins

Viral Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 1409697
DOI: 10.1073/pnas.89.20.9774
PMCID: PMC50215

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1409697-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Genetic analysis of the interaction between bacteriophage T7 DNA polymerase and Escherichia coli thioredoxin.PMID 1409697Read ↗

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