Article
A single-stranded DNA-binding protein of bacteriophage T7 defective in DNA annealing.
The Journal of biological chemistry - 1 Aug 2003
Rezende Lisa F, Willcox Smaranda, Griffith Jack D, Richardson Charles C
Abstract excerpt
The annealing of complementary strands of DNA is a vital step during the process of DNA replication, recombination, and repair. In bacteriophage T7-infected cells, the product of viral gene 2.5, a single-stranded DNA-binding protein, performs this function. We have identified a single amino acid residue in gene 2.5 protein, arginine 82, that is critical for its DNA annealing activity. Expression of gene 2.5...
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