Article
High-throughput genotyping by coupling adapter-ligation mediated allele-specific amplification with microplate array parallel gel electrophoresis.
Molecular biotechnology - 1 Jan 2010
Wang Weipeng, Zhang Xiaodan, Zhou Guohua
Abstract excerpt
Recently, we have developed a method of adapter-ligation mediated allele-specific amplification (ALM-ASA) for simultaneously typing multiple single nucleotide polymorphisms (SNPs) at a low cost. We usually use agarose gel-electrophoresis for analyzing PCR products. As the processes of sampling and PCR can be carried out at a format of 96-well or 384-well, the throughput-bottleneck of whole process of ALM-ASA is...
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