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Article

Accurate determination of microbial diversity from 454 pyrosequencing data.

Nature methods - 1 Sept 2009

Quince Christopher, Lanzén Anders, Curtis Thomas P, Davenport Russell J, Hall Neil, Head Ian M, Read L Fiona, Sloan William T

Abstract excerpt

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it...

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Identifiers

PMID: 19668203
DOI: 10.1038/nmeth.1361

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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