Article
De novo semi-alignment of 16S rRNA gene sequences for deep phylogenetic characterization of next generation sequencing data.
Microbes and environments - 1 Jan 2013
Avershina Ekaterina, Frisli Trine, Rudi Knut
Abstract excerpt
We addressed the challenges of analyzing next-generation 16S rRNA gene deep sequencing data from the uncharacterized microbial majority. This was performed using a novel de novo semi-alignment approach. The semi-alignments were based on Orthologous Tri-Nucleotides (OTNs), which are identical trinucleotides located in the same sequence region. OTNs in high error homopolymeric tracts were excluded to avoid...
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