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Article

PyroClean: denoising pyrosequences from protein-coding amplicons for the recovery of interspecific and intraspecific genetic variation.

PloS one - 1 Jan 2013

Ramirez-Gonzalez Ricardo, Yu Douglas W, Bruce Catharine, Heavens Darren, Caccamo Mario, Emerson Brent C

Abstract excerpt

High-throughput parallel sequencing is a powerful tool for the quantification of microbial diversity through the amplification of nuclear ribosomal gene regions. Recent work has extended this approach to the quantification of diversity within otherwise difficult-to-study metazoan groups. However, nuclear ribosomal genes present both analytical challenges and practical limitations that are a consequence of the...

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PMID: 23469211
DOI: 10.1371/journal.pone.0057615
PMCID: PMC3585932

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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