Article
Tandem DNA-bound cAMP-CRP complexes are required for transcriptional repression of the deoP2 promoter by the CytR repressor in Escherichia coli.
Molecular microbiology - 1 Sept 1990
Søgaard-Andersen L, Møllegaard N E, Douthwaite S R, Valentin-Hansen P
Abstract excerpt
We have studied the deoP2 promoter in Escherichia coli to define features important for its interaction with the CytR repressor. As is characteristic for CytR-regulated promoters, deoP2 encodes tandem binding sites for the activating complex cAMP-CRP. One of these sites, CRP-1, overlaps the -35 region, and is sufficient for activation; the second site, CRP-2, centred around -93, is indispensable for repression....
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Cyclic AMP
- Cyclic AMP Receptor Protein
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Molecular Sequence Data