Article
Protein-protein communication: structural model of the repression complex formed by CytR and the global regulator CRP.
Cell - 27 Jun 1997
Kallipolitis B H, Nørregaard-Madsen M, Valentin-Hansen P
Abstract excerpt
The cAMP receptor protein (CRP) and the LacI-related CytR antiactivator bind cooperatively to adjacent DNA sites at or near promoters, an interaction that involves direct protein contacts. Here, we identify a collection of amino acid substitutions in CytR that reestablish protein-protein communic...
Topics
- Alanine
- Alleles
- Amino Acid Sequence
- Bacterial Proteins
- Carrier Proteins
- Cells, Cultured
- Cyclic AMP Receptor Protein
- DNA
- DNA-Binding Proteins
- Dimerization
- Escherichia coli
- Escherichia coli Proteins
- Molecular Sequence Data
- Mutagenesis
- Protein Conformation
- Protein Structure, Tertiary
- Repressor Proteins
- Sensitivity and Specificity