Article
[The effects of genes ste7-stel5 double disruption in ebosin biosynthesis].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Apr 2009
Bai Liping, Jiang Rong, Shan Junjie, Guo Lianhong, Zhang Yang, Li Yuan
Abstract excerpt
OBJECTIVE: To study the effects of genes ste7-ste15 double disruption in biosynthesis of Ebosin. METHODS: The ste7 gene was disrupted with a double crossover via homologous recombination in the mutant strain Streptomyces sp. The 139 (ste15-) and the mutant strain Streptomyces sp.139 (ste7- ste15-) were identified by Southern blot. Gene complementation of the knock-out mutant was done. Monosaccharide composition...
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