Article
[Construction of Saccharomyces cerevisiae mutant with knockout of SNF4 gene].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Apr 2011
Lin Xiaohua, Ke Chongrong, Wu Bisha, Zheng Yongbiao, Li Li, Chen Youqiang, Huang Jianzhong
Abstract excerpt
Construction and ethanol production effects of SNF4 gene knockout in Saccharomyces cerevisiae were described in this paper. For knockout of SNF4 gene in S. cerevisiae YS2, a PCR-amplified disruption cassette was used, encoding the short flanking homologous regions to the SNF4 gene and Kan(r) as selectable marker. The SNF4 gene disruption cassette was transformed into S. cerevisiae YS2 through LiAc/SS Carrier...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
