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Article

Intramembrane glycine mediates multimerization of Insig-2, a requirement for sterol regulation in Chinese hamster ovary cells.

Journal of lipid research - 1 Jan 2010

Lee Peter C W, DeBose-Boyd Russell A

Abstract excerpt

Sterol-induced binding of endoplasmic reticulum (ER) membrane proteins Insig-1 and Insig-2 to SREBP cleavage-activating protein (Scap) and HMG-CoA reductase triggers regulatory events that limit cholesterol synthesis in animal cells. Binding of Insigs to Scap prevents proteolytic activation of sterol-regulatory element binding proteins (SREBPs), membrane-bound transcription factors that enhance cholesterol...

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Identifiers

PMID: 19617589
DOI: 10.1194/jlr.M900336-JLR200
PMCID: PMC2789779

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Research trail→

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Intramembrane glycine mediates multimerization of Insig-2, a requirement for sterol regulation in Chinese hamster ovary cells.PMID 19617589Read ↗

Isolation of sterol-resistant Chinese hamster ovary cells with genetic deficiencies in both Insig-1 and Insig-2.Semantic proximity Read ↗

Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.Semantic proximity Read ↗

Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.Semantic proximity Read ↗

Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterols.Semantic proximity Read ↗

Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.Semantic proximity Read ↗

Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro.Semantic proximity Read ↗

Mutant mammalian cells as tools to delineate the sterol regulatory element-binding protein pathway for feedback regulation of lipid synthesis.Semantic proximity Read ↗

An autonomous, but INSIG-modulated, role for the sterol sensing domain in mallostery-regulated ERAD of yeast HMG-CoA reductase.Semantic proximity Read ↗

Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins.Semantic proximity Read ↗

Dislocation of HMG-CoA reductase and Insig-1, two polytopic endoplasmic reticulum proteins, en route to proteasomal degradation.Semantic proximity Read ↗

Proteasomal degradation of ubiquitinated Insig proteins is determined by serine residues flanking ubiquitinated lysines.Semantic proximity Read ↗

A structure of human Scap bound to Insig-2 suggests how their interaction is regulated by sterols.Semantic proximity Read ↗

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