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Article

Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.

Cell - 23 Aug 2002

Yang Tong, Espenshade Peter J, Wright Michael E, Yabe Daisuke, Gong Yi, Aebersold Ruedi, Goldstein Joseph L, Brown Michael S

Abstract excerpt

Using coimmunoprecipitation and tandem mass spectrometry, we identify INSIG-1 as an ER protein that binds the sterol-sensing domain of SREBP cleavage-activating protein (SCAP) and facilitates retention of the SCAP/SREBP complex in the ER. In sterol-depleted cells, SCAP escorts SREBPs from ER to Golgi for proteolytic processing, thereby allowing SREBPs to stimulate cholesterol synthesis. Sterols induce binding of...

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Source

Identifiers

PMID: 12202038
DOI: 10.1016/s0092-8674(02)00872-3

Genes

SCAP

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER.PMID 12202038Read ↗

Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.Semantic proximity Read ↗

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A structure of human Scap bound to Insig-2 suggests how their interaction is regulated by sterols.Semantic proximity Read ↗

Mutant mammalian cells as tools to delineate the sterol regulatory element-binding protein pathway for feedback regulation of lipid synthesis.Semantic proximity Read ↗

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