Article
Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterols.
Proceedings of the National Academy of Sciences of the United States of America - 24 Dec 2002
Yabe Daisuke, Xia Zong-Ping, Adams Christopher M, Rawson Robert B
Abstract excerpt
We report the isolation and characterization of a new line of mutant Chinese hamster ovary cells, designated SRD-5, that are resistant to 25HC, a potent suppressor of cleavage of sterol regulatory element-binding proteins (SREBPs) in mammalian cells. In SRD-5 cells, SREBPs are cleaved constitutively, generating transcriptionally active nuclear SREBP even in the presence of sterols. Sequence analysis of SREBP...
Topics
- Amino Acid Sequence
- Animals
- CCAAT-Enhancer-Binding Proteins
- CHO Cells
- Carrier Proteins
- Cricetinae
- DNA-Binding Proteins
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Mice
- Molecular Sequence Data
- Mutation
- Sterol Regulatory Element Binding Protein 1