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Article

Identification of amino acid residues of the pheromone-binding domain of the transcription factor TraR that are required for positive control.

Molecular microbiology - 1 Aug 2009

Costa Esther D, Cho Hongbaek, Winans Stephen C

Abstract excerpt

Genes required for replication and for conjugal transfer of the Agrobacterium tumefaciens Ti plasmid are regulated by the quorum sensing transcription factor TraR, whose N-terminal domain binds to the pheromone 3-oxo-octanoylhomoserine lactone (OOHL) and whose C-terminal domain binds to specific DNA sequences called tra boxes. Here, we constructed 117 mutants, altering 103 surface-exposed amino acid residues of...

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Source

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Identifiers

PMID: 19602141
DOI: 10.1111/j.1365-2958.2009.06755.x
PMCID: PMC2748755

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

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Identification of amino acid residues of the pheromone-binding domain of the transcription factor TraR that are required for positive control.PMID 19602141Read ↗

Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription.Semantic proximity Read ↗

Domains formed within the N-terminal region of the quorum-sensing activator TraR are required for transcriptional activation and direct interaction with RpoA from agrobacterium.Semantic proximity Read ↗

N- and C-terminal regions of the quorum-sensing activator TraR cooperate in interactions with the alpha and sigma-70 components of RNA polymerase.Semantic proximity Read ↗

The quorum sensing transcriptional regulator TraR has separate binding sites for DNA and the anti-activator.Semantic proximity Read ↗

The antiactivator TraM interferes with the autoinducer-dependent binding of TraR to DNA by interacting with the C-terminal region of the quorum-sensing activator.Semantic proximity Read ↗

Amino-terminal protein fusions to the TraR quorum-sensing transcription factor enhance protein stability and autoinducer-independent activity.Semantic proximity Read ↗

Quorum-sensing antiactivator TraM forms a dimer that dissociates to inhibit TraR.Semantic proximity Read ↗

Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ.Semantic proximity Read ↗

Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer.Semantic proximity Read ↗

Most mutant OccR proteins that are defective in positive control hold operator DNA in a locked high-angle bend.Semantic proximity Read ↗

Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10.Semantic proximity Read ↗

The Agrobacterium tumefaciens rnd homolog is required for TraR-mediated quorum-dependent activation of Ti plasmid tra gene expression.Semantic proximity Read ↗

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