Article
Domains formed within the N-terminal region of the quorum-sensing activator TraR are required for transcriptional activation and direct interaction with RpoA from agrobacterium.
The Journal of biological chemistry - 24 Sept 2004
Qin Yinping, Luo Zhao-Qing, Farrand Stephen K
Abstract excerpt
TraR, a quorum-sensing activator, induces transcription from its binding site, the tra-box, located upstream of Ti plasmid target promoters. TraR activated expression of a lacZ reporter in Escherichia coli only when RpoAAt from Agrobacterium tumefaciens was co-expressed. As assessed by gel retardation assays RpoAAt, but not RpoAEc, formed a ternary complex with TraR and a tra-box probe in vitro. TraR formed...
Topics
- Agrobacterium tumefaciens
- Alleles
- Bacterial Proteins
- DNA
- DNA-Directed RNA Polymerases
- Dimerization
- Escherichia coli
- Genes, Dominant
- Genes, Reporter
- Genetic Complementation Test
- Kinetics
- Models, Molecular
- Mutation