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Article

Expression and purification of soluble HIV-1 envelope glycoprotein gp160 mutant from Saccharomyces cerevisiae.

Journal of bioscience and bioengineering - 1 Jul 2009

Yang Kunyu, Li Shaowei, He Fangping, Zhang Jiannan, Wei Minxi, Pan Huirong, Yang Chunyan, Xiong Junhui, Zhang Jun, Xia Ningshao

Abstract excerpt

Here we report the expression of HIV-1 gp160 and its mutated proteins in Saccharomyces cerevisiae. Two strong hydrophobic regions, aa 511-537 and aa 679-703, were predicted by GCG Wisconsin Package software and removed to investigate the solubility of the mutated gp160 (gp160Delta12). The results showed that gp160Delta12 assumes high solubility as to be present in supernatant of cell lysate exclusively. The...

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PMID: 19577184
DOI: 10.1016/j.jbiosc.2009.02.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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