Article
Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles.
1 Aug 1997
Abstract excerpt
We have expressed cDNA encoding the dengue virus structural proteins in Pichia pastoris by chromosomal integration of an expression cassette containing the dengue virus structural genes (CprME). The yeast recombinant E protein migrated during SDS-PAGE as a 65 kDa protein when analysed by Western blotting and radioimmunoprecipitation, which is the expected molecular mass for correctly processed and glycosylated E...
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