Article
Cloning and expression of the external-glycoprotein gene mutant from HIV-2 in the methylotrophic yeast Pichia pastoris and identification of the glycoprotein.
Biotechnology and applied biochemistry - 1 Aug 2001
Zhang Y J, Jin N Y, Jiang W Z, Zhu X J, Shen J C
Abstract excerpt
To achieve high-level expression of HIV-2(ROD) external glycoprotein gp105 in Pichia pastoris, the gp105 gene mutant tP1, with the 5' non-functional region of the gp105 gene removed, was obtained by PCR amplification and was cloned into secreted expression vector pHILS1. The His(+)Mut(s) recombin...
Topics
- Blotting, Western
- Cloning, Molecular
- Codon
- Electrophoresis, Polyacrylamide Gel
- Gene Products, env
- Glycoproteins
- HIV-2
- Histidine
- Mutation
- Pichia
- Plasmids
- Polymerase Chain Reaction
- Recombinant Proteins
- env Gene Products, Human Immunodeficiency Virus