Article
Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae.
Molecular cell - 26 Jun 2009
Azvolinsky Anna, Giresi Paul G, Lieb Jason D, Zakian Virginia A
Abstract excerpt
Replication forks face multiple obstacles that slow their progression. By two-dimensional gel analysis, yeast forks pause at stable DNA protein complexes, and this pausing is greatly increased in the absence of the Rrm3 helicase. We used a genome-wide approach to identify 96 sites of very high DNA polymerase binding in wild-type cells. Most of these binding sites were not previously identified pause sites....
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