Article
A synthetic protein selected for ligand binding affinity mediates ATP hydrolysis.
ACS chemical biology - 21 Aug 2009
Simmons Chad R, Stomel Joshua M, McConnell Michael D, Smith Daniel A, Watkins Jennifer L, Allen James P, Chaput John C
Abstract excerpt
How primitive enzymes emerged from a primordial pool remains a fundamental unanswered question with important practical implications in synthetic biology. Here we show that a de novo evolved ATP binding protein, selected solely on the basis of its ability to bind ATP, mediates the regiospecific hydrolysis of ATP to ADP when crystallized with 1 equiv of ATP. Structural insights into this reaction were obtained by...
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