Article
Detection of the TLR4 1196C>T polymorphism by mismatched-polymerase chain reaction using plasmid DNA as internal control in restriction fragment length polymorphism assays.
Genetic testing and molecular biomarkers - 1 Jun 2009
Lima-Neto Lidio G, Hirata Rosario D, Luchessi Andre D, Silbiger Vivian N, Pastorelli Carla P, Sampaio Marcelo F, Armaganijan Dikran, Rezende Adriana A, Doi Sonia Q, Hirata Mario H
Abstract excerpt
BACKGROUND: Restriction fragment length polymorphism (RFLP) is a common molecular assay used for genotyping, and it requires validated quality control procedures to prevent mistyping caused by impaired endonuclease activity. We have evaluated the usefulness of a plasmid-based internal control in RFLP assays. RESULTS: Blood samples were collected from 102 individuals with acute myocardial infarction (AMI) and 108...
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