Article
Development of an internal restriction control in the PCR detection of the methylenetetrahydrofolate reductase (MTHFR) C677T mutation.
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology - 1 Jun 1999
Lastrucci R M, Dawson D A, Münster M
Abstract excerpt
BACKGROUND: Detection of the presence of 677C/T allele in the human MTHFR gene is easily achieved by amplification using primers designed to span this region. Hinf I digestion, occurring only in the 677T allele, subsequently discriminates between the two alleles. Existing methods, however, do not control for failed restriction endonuclease digestion. METHODS AND RESULTS: A new forward, modified primer was...
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