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primers4clades: a web server that uses phylogenetic trees to design lineage-specific PCR primers for metagenomic and diversity studies.

Nucleic acids research - 1 Jul 2009

Contreras-Moreira Bruno, Sachman-Ruiz Bernardo, Figueroa-Palacios Iraís, Vinuesa Pablo

Abstract excerpt

Primers4clades is an easy-to-use web server that implements a fully automatic PCR primer design pipeline for cross-species amplification of novel sequences from metagenomic DNA, or from uncharacterized organisms, belonging to user-specified phylogenetic clades or taxa. The server takes a set of non-aligned protein coding genes, with or without introns, aligns them and computes a neighbor-joining tree, which is...

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PMID: 19465390
DOI: 10.1093/nar/gkp377
PMCID: PMC2703966

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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