Article

DeGenPrime provides robust primer design and optimization unlocking the biosphere

2023-08-14

Abstract excerpt

Polymerase chain reaction (PCR) is the world’s most important molecular diagnostic with applications ranging from medicine to ecology. PCR can fail because of poor primer design. The nearest-neighbor thermodynamic properties, picking conserved regions, and filtration via penalty of oligonucleotides form the basis for good primer design. DeGenPrime is a console-based high quality PCR primer design tool that can uti...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f09a39e0-29a3-5f70-9477-0e1b146cb775
DOI: 10.1101/2023.08.11.553048

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

DeGenPrime provides robust primer design and optimization unlocking the biosphereDOI 10.1101/2023.08.11.553048Read

MultiPrime: A Reliable and Efficient Tool for Targeted Next-Generation SequencingSemantic proximity Read

PCR-SSP: a cautionary tale and an update of the Bein primer mixes.Semantic proximity Read

PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.Semantic proximity Read

primerJinn – a tool for rationally designing multiplex PCR primer sets and in silico PCRSemantic proximity Read

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCRSemantic proximity Read

URPD: a specific product primer design tool.Semantic proximity Read

PrimalScheme: open-source community resources for low-cost viral genome sequencingSemantic proximity Read

Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of AlignmentsSemantic proximity Read

DiPPER2 – a user-friendly pipeline for picking and evaluating taxon-specific PCR primersSemantic proximity Read

varVAMP: degenerate primer design for tiled full genome sequencing and qPCRSemantic proximity Read

Scaling Variant-Aware Multiplex Primer DesignSemantic proximity Read

RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation.Semantic proximity Read