Article
PrimerSNP: a web tool for whole-genome selection of allele-specific and common primers of phylogenetically-related bacterial genomic sequences.
BMC microbiology - 20 Oct 2008
Yao Jiqiang, Lin Hong, Van Deynze Allen, Doddapaneni Harshavardhan, Francis Martha, Lemos Eliana Gertrudes Macedo, Civerolo Edwin L
Abstract excerpt
BACKGROUND: The increasing number of genomic sequences of bacteria makes it possible to select unique SNPs of a particular strain/species at the whole genome level and thus design specific primers based on the SNPs. The high similarity of genomic sequences among phylogenetically-related bacteria requires the identification of the few loci in the genome that can serve as unique markers for strain differentiation....
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