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Article

Measurement of cellular beta-site of APP cleaving enzyme 1 activity and its modulation in neuronal assay systems.

Analytical biochemistry - 15 Apr 2009

Volbracht Christiane, Penzkofer Stephan, Mansson David, Christensen Kenneth Vielsted, Fog Karina, Schildknecht Stefan, Leist Marcel, Nielsen Jacob

Abstract excerpt

Amyloid-beta peptide (Abeta), a putatively causative agent of Alzheimer's disease (AD), is proteolytically derived from beta-amyloid precursor protein (APP). Here we describe cellular assays to detect the activity of the key protease beta-site of APP cleaving enzyme 1 (BACE1) based on an artificial reporter construct containing the BACE1 cleavage site of APP. These methods allow identification of inhibitors and...

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Source

Identifiers

PMID: 19454261
DOI: 10.1016/j.ab.2009.01.008

Genes

APP
BACE1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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