Article
Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis.
Molecular microbiology - 1 Jun 2009
Daubenspeck James M, Bolland Jeffrey R, Luo Wenyi, Simmons Warren L, Dybvig Kevin
Abstract excerpt
The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal beta-linked galactose residues. Mutants that failed to produce the EPS bound by GS-I were...
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