Efficient genotyping of Schistosoma mansoni miracidia following whole genome amplification.

doi:10.1016/j.molbiopara.2009.02.010

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Article

Efficient genotyping of Schistosoma mansoni miracidia following whole genome amplification.

Molecular and biochemical parasitology - 1 Jul 2009

Valentim Claudia L L, LoVerde Philip T, Anderson Timothy J C, Criscione Charles D

Abstract excerpt

Small parasites and larval stages pose a problem for molecular analyses because limited amounts of DNA template are available. Isothermal methods for faithfully copying DNA have the potential to revolutionize studies of such organisms. We evaluated the fidelity of multiple displacement amplification (MDA) for amplifying DNA extracted from a single miracidium of Schistosoma mansoni. To do this we genotyped DNA...

Topics

Animals
Female
Genetic Techniques
Genome, Helminth
Genotype
Life Cycle Stages
Male
Microsatellite Repeats
Reproducibility of Results
Schistosoma mansoni

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Identifiers

PMID: 19428677
DOI: 10.1016/j.molbiopara.2009.02.010
PMCID: PMC2771185

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