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Optimal sample storage and extraction procotols for reliable multilocus genotyping of the human parasite Schistosoma mansoni.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Aug 2011

Van den Broeck F, Geldof S, Polman K, Volckaert F A M, Huyse T

Abstract excerpt

Genotyping individual larval stages and eggs of natural parasite populations is complicated by the difficulty of obtaining reliable genotypes from low quantity DNA template. A suitable storage and extraction protocol, together with a thorough quantification of genotyping errors are therefore crucial for molecular epidemiological studies. Here we test the robustness, handling time, ease of use, cost effectiveness...

Topics

Animals
DNA, Helminth
Feces
Genotype
Humans
Microsatellite Repeats
Multilocus Sequence Typing
Polymerase Chain Reaction
Schistosoma mansoni
Schistosomiasis mansoni

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Specimen Handling

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Identifiers

PMID: 21605705
DOI: 10.1016/j.meegid.2011.05.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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