Article
Use of multiple displacement amplification to increase the detection and genotyping of trypanosoma species samples immobilized on FTA filters.
The American journal of tropical medicine and hygiene - 1 Jun 2007
Morrison Liam J, McCormack Gillian, Sweeney Lindsay, Likeufack Anne C L, Truc Philippe, Turner C Michael, Tait Andy, MacLeod Annette
Abstract excerpt
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias. Multiple displacement amplification (MDA) amplifies DNA with a simple in vitro step and was evaluated on mouse blood samples on FTA filter cards with known numbers of Trypanosoma brucei parasites. The data showed a...
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