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A fast method to diagnose chromosome and plasmid loss in Saccharomyces cerevisiae strains.

Yeast (Chichester, England) - 1 Jul 1999

Hegemann J H, Klein S, Heck S, Güldener U, Niedenthal R K, Fleig U

Abstract excerpt

We have developed a simple, fast and reliable method for the analysis of genetic stability in budding yeast strains. The assay relies on our previous finding that cells expressing the green fluorescent protein (GFP) can be detected and counted by flow cytometric analysis (FACS) (Niedenthal et al., 1996). Expression of a gfp-carrying CEN-plasmid in a wild-type strain resulted in the emission of strong fluorescence...

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Source

Identifiers

PMID: 10407280
DOI: 10.1002/(SICI)1097-0061(199907)15:10B<1009::AID-YEA396>3.0.CO;2-I

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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