Article
Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3' splice acceptor site.
The EMBO journal - 1 Dec 1991
Huang J, Van der Ploeg L H
Abstract excerpt
We studied sequence requirements for trans-splicing at the 3' splice acceptor site of a procyclic acidic repetitive protein (PARP) coding gene in trypanosomes. In transient CAT transfection assays with linker scanning (LS) mutants in a PARP promoter--3' splice acceptor site--CAT construct, minor...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Chloramphenicol O-Acetyltransferase
- Membrane Glycoproteins
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Protozoan Proteins
- Pyrimidines
- RNA Splicing
- RNA, Messenger
- RNA, Protozoan
- Transformation, Genetic
- Trypanosoma
- Variant Surface Glycoproteins, Trypanosoma