Article
A high-throughput assay using liquid chromatography-tandem mass spectrometry for simultaneous in vivo phenotyping of 5 major cytochrome p450 enzymes in patients.
Therapeutic drug monitoring - 1 Apr 2009
Ghassabian Sussan, Chetty Manoranjenni, Tattam Bruce N, Chem Mraci C, Glen John, Rahme Jeannie, Stankovic Zvijezdana, Ramzan Iqbal, Murray Michael, McLachlan Andrew J
Abstract excerpt
The phenotyping cocktail is a practical approach for phenotyping of cytochrome P450 (CYP) enzymes in vivo. In this study, a liquid chromatography-tandem mass spectrometry method using a dual-extraction approach was developed and validated to quantify 5 selective substrates and their metabolites for the simultaneous phenotyping CYPs 1A2, 2C19, 2C9, 2D6, and 3A4 in patient blood samples. The assay was applied in a...
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