Article
Quadruple-allele chemiluminometric assay for simultaneous genotyping of two single-nucleotide polymorphisms.
The Analyst - 1 Apr 2009
Elenis Dimitrios S, Ioannou Penelope C, Christopoulos Theodore K
Abstract excerpt
We developed a rapid, simple, cost-effective and high sample-throughput method for the simultaneous detection of four alleles in single-nucleotide polymorphisms (SNPs). The method was applied to the simultaneous genotyping of two common SNPs within the TLR4 gene, the A896G and C1196T polymorphisms. The method consists of a single PCR of the region spanning the A896G and C1196T polymorphic sites, followed by a...
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