Article
Dual-allele dipstick assay for genotyping single nucleotide polymorphisms by primer extension reaction.
European journal of human genetics : EJHG - 1 Jan 2009
Konstantou Jessica K, Ioannou Penelope C, Christopoulos Theodore K
Abstract excerpt
We have developed a dry-reagent dipstick test for simultaneous visual detection of two alleles in single nucleotide polymorphisms (SNPs). The strip comprises two test zones and a control zone. Oligonucleotide-functionalized gold nanoparticles are used as reporters. PCR-amplified DNA that spans the interrogated sequence is subjected to primer extension (PEXT) reactions using allele-specific primers....
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