Article
Regulation of EcoRII methyltransferase: effect of mutations on gene expression and in vitro binding to the promoter region.
Nucleic acids research - 11 Dec 1994
Som S, Friedman S
Abstract excerpt
EcoRII Methyltransferase (M.EcoRII) which methylates the second C in the sequence CCWGG (W = A/T) is autogenously regulated by binding to the 5' regulatory region of its gene. DNase I footprinting experiments demonstrated that purified M.EcoRII protected a 47-49 bp region of DNA immediately upstr...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA
- DNA-Cytosine Methylases
- Enzyme Repression
- Gene Expression Regulation, Enzymologic
- Lac Operon
- Methylation
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- Recombinant Fusion Proteins
- beta-Galactosidase