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Article

Regulation of EcoRII methyltransferase: effect of mutations on gene expression and in vitro binding to the promoter region.

Nucleic acids research - 11 Dec 1994

Som S, Friedman S

Abstract excerpt

EcoRII Methyltransferase (M.EcoRII) which methylates the second C in the sequence CCWGG (W = A/T) is autogenously regulated by binding to the 5' regulatory region of its gene. DNase I footprinting experiments demonstrated that purified M.EcoRII protected a 47-49 bp region of DNA immediately upstream of the ecoRIIM coding region. We have studied this interaction with mutants of the enzyme, in vitro by DNA binding...

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Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Promoter Regions, Genetic

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Source

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Identifiers

PMID: 7816624
DOI: 10.1093/nar/22.24.5347
PMCID: PMC332081

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Regulation of EcoRII methyltransferase: effect of mutations on gene expression and in vitro binding to the promoter region.PMID 7816624Read ↗

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