Article
The cysteine conserved among DNA cytosine methylases is required for methyl transfer, but not for specific DNA binding.
Nucleic acids research - 25 Jan 1993
Wyszynski M W, Gabbara S, Kubareva E A, Romanova E A, Oretskaya T S, Gromova E S, Shabarova Z A, Bhagwat A S
Abstract excerpt
All DNA (cytosine-5)-methyltransferases contain a single conserved cysteine. It has been proposed that this cysteine initiates catalysis by attacking the C6 of cytosine and thereby activating the normally inert C5 position. We show here that substitutions of this cysteine in the E. coli methylase M. EcoRII with either serine or tryptophan results in a complete loss of ability to transfer methyl groups to DNA....
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