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Article

Rational design of a minimal and highly enriched CYP102A1 mutant library with improved regio-, stereo- and chemoselectivity.

Chembiochem : a European journal of chemical biology - 23 Mar 2009

Seifert Alexander, Vomund Sandra, Grohmann Katrin, Kriening Sebastian, Urlacher Vlada B, Laschat Sabine, Pleiss Jürgen

Abstract excerpt

A minimal CYP102A1 mutant library of only 24 variants plus wild type was constructed by combining five hydrophobic amino acids (alanine, valine, phenylalanine, leucine and isoleucine) in two positions. Both positions are located close to the centre of the haem group. The first, position 87, has been shown to mediate substrate specificity and regioselectivity in CYP102A1. The second hotspot, position 328, was...

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PMID: 19222039
DOI: 10.1002/cbic.200800799

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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