Article
Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technology.
Clinical chemistry - 1 Dec 2002
Santacroce Rosa, Ratti Antonia, Caroli Francesco, Foglieni Barbara, Ferraris Alessandro, Cremonesi Laura, Margaglione Maurizio, Seri Marco, Ravazzolo Roberto, Restagno Gabriella, Dallapiccola Bruno, Rappaport Eric, Pollak Eleanor S, Surrey Saul, Ferrari Maurizio, Fortina Paolo
Abstract excerpt
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We developed methods for detection of single-nucleotide polymorphisms (SNPs) in clinically relevant genes. METHODS: Primer pairs, with one containing a 5'-biotin group, were used to PCR-amplify the region encompassing the SNP to be interrogated. After denaturation, the biotinylated strand was electronically targeted to...
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