Article
Methods of plate pexophagy monitoring and positive selection for ATG gene cloning in yeasts.
Methods in enzymology - 1 Jan 2008
Stasyk Oleh V, Nazarko Taras Y, Sibirny Andriy A
Abstract excerpt
Methods for colony assay of peroxisomal oxidases in yeasts provide a convenient and fast approach for monitoring peroxisome status. They have been used in several laboratories for the isolation of yeast mutants deficient in selective autophagic peroxisome degradation (pexophagy), catabolite repression of peroxisomal enzymes or mutants deficient in oxidases themselves. In this chapter, protocols for monitoring...
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