Article
An efficient screen for peroxisome-deficient mutants of Pichia pastoris.
Journal of bacteriology - 1 Aug 1992
Liu H, Tan X, Veenhuis M, McCollum D, Cregg J M
Abstract excerpt
We describe a rapid and efficient screen for peroxisome-deficient (per) mutants in the yeast Pichia pastoris. The screen relies on the unusual ability of P. pastoris to grow on two carbon sources, methanol and oleic acid, both of which absolutely require peroxisomes to be metabolized. A collectio...
Topics
- Acyl-CoA Oxidase
- Alcohol Oxidoreductases
- Methanol
- Microbodies
- Mutation
- Oleic Acid
- Oleic Acids
- Oxidoreductases
- Pichia