Engineered cysteine mutants of myosin light chain 2. Fluorescent analogues for structural studies.

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Article

Engineered cysteine mutants of myosin light chain 2. Fluorescent analogues for structural studies.

The Journal of biological chemistry - 15 Oct 1991

Saraswat L D, Lowey S

Abstract excerpt

Site-directed mutagenesis has been used to insert cysteine residues at specific locations in the myosin light chain 2 (LC2) sequence. The aim was to modify these cysteines with one or more spectroscopic probes and to reconstitute myosin with labeled light chains for structural studies. Native LC2 has two endogenous cysteine residues at positions 126 and 155; a third sulfhydryl was added by replacing either Pro2,...

Topics

Amino Acid Sequence
Animals
Antibodies
Base Sequence
Chickens
Chromatography, Liquid
Cysteine
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fluorescein
Fluoresceins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Fluorescent Dyes

Genetic Engineering

Microscopy, Electron

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Myosins

Source

Open in PubMed

Identifiers

PMID: 1918082

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1918082-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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